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    1. Get out of the cold & warm up to new Gibco BenchStable Media! Optimized for storage at room temperature »

      RPMI 1640 Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

      Catalog number:  11875119

       Related applications:

      Mammalian Cell Culture

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      RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

      This RPMI is modified as follows:
      • L-glutamine• HEPES
      • Phenol Red

      The complete formulation is available.

      Using RPMI
      RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

      Product use
      For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

      cGMP manufacturing and quality system
      RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (21875-091). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
      For In Vitro Diagnostic Use.


      Glutamine: L-Glutamine
      Phenol Red Indicator: Phenol Red
      Form: Liquid
      HEPES Buffer: No HEPES
      Serum Supplementation: Standard Serum Supplementation
      Sodium Pyruvate Additive: No Sodium Pyruvate
      Product Line: Gibco®
      Product Size: 10 x 500 mL
      Volume (Metric): 500 ml
      Green Features: Sustainable packaging
      Shipping Condition: Room Temperature

      Contents & storage

      Storage conditions: 2°C to 8°C (protect from light)
      Shipping conditions: Ambient
      Shelf life: 12 months from date of manufacture

      Media formulations


      Manuals & protocols

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